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Culture Methodology for Legionella Species
by Janet E. Stout, Ph.D.

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Contents

About the author

Price and ordering information

 

 

Special report available in electronic format, 1998, 14 pages

Written for laboratorians, this report provides a guide for Legionella culture of clinical  specimens and environmental samples.

You can access this electronic document immediately after you order, or at your convenience within five days after you order. 

The document is in PDF format, which can be viewed with Adobe Acrobat Reader. If you don't already have Adobe Acrobat Reader on your computer, you can download for free at www.adobe.com.

Contents  

Clinical Specimens 

  • Storage and transport
  • Media
Environmental Samples 
  • Storage temperature
  • Processing samples collected from water distribution systems
  • Processing samples collected from cooling towers and other equipment with high concentrations of bacteria
  • Media for samples collected from water distribution systems
  • Media for samples collected from cooling towers and other equipment with high concentrations of bacteria
Figures and Tables 
  • Procedure for processing clinical samples for Legionella culture
  • Acid-buffer pre-treatment procedures
  • Procedure for processing water samples for Legionella culture
  • Three currently recommended methods for monitoring Legionella pneumophila in potable water
Appendix: Suppliers of Legionella media and filters

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About the author

Janet E. Stout, Ph.D., is Director of the Special Pathogens Laboratory at the VA Medical Center in Pittsburgh (USA) and a Research Assistant Professor at the University of Pittsburgh School of Medicine. She earned her BS in Biology from Clarion (Pennsylvania) State College and her Masters and Ph.D. degrees in Microbiology from the University of Pittsburgh. Dr. Stout has significant experience in Legionella detection and disinfection. Recent studies have involved eradication of legionellae from hospital water systems by copper-silver ionization, molecular epidemiology of Legionnaires' disease, and legionellae in biofilms. She is the author of three book chapters on Legionnaires' disease and more than 40 peer-reviewed papers, including papers published in The New England Journal of Medicine, Journal of the American Medical Association, Journal of Clinical Microbiology, and the Journal of Infectious Diseases. Dr. Stout is a member of the American Society for Microbiology and Society for Healthcare Epidemiology of American. 

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Price and ordering information

Paper version, Item 308 SOLD OUT. PLEASE USE  ELECTRONIC VERSION.

Electronic version, Item 308e, $13
Please contact us about a multi-user license.

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You can access the electronic version immediately after you order, or at your convenience within five days after you order.  You can save it on your computer, and also print it. The document is in PDF format, which can be viewed with Adobe Acrobat Reader. If you don't already have Adobe Acrobat Reader on your computer, you can download for free at www.adobe.com.

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